Discovery of antagonistic Synechococcus-heterotrophic bacteria interactions

We have successfully developed a protocol for growing co-cultures of heterotrophic bacteria andcyanobacteria. We tested phylogenetically diverse heterotrophic bacteria (Vibrio, Pseudoalteromonas, Alteromonas, Flavobacterium) with two cyanobacteria, one belonging to the coastal clade (CC9311) and the other to the oceanic clade (WH8102). Our data indicate that SWAT3 (Vibrio), ATW7 (Alteromonas),Pseudoalteromonas flavipulchra (Pesudoalteromonas) strongly inhibit the growth of CC9311 and WH8102 whereas BBFL7 (Flavobacterium) doesn’t. This suggests that some Synechococcus-bacteria associations are antagonistic in nature. Thus, some bacteria may regulate Synechococcus-based primary production as well as contribute to variations in clade-specific productivity. These are important findings and we are currently elucidating the mechanism by which bacteria inhibit/kill Synechococcus.

                                      Figure 1: Co-culture growth effect experiments among WH8102 and CC9311 Synechococcus strains and heterotrophic bacteria in SN medium.

Other Project Data

Bacteria-cyanobacteria associations are phylogenetically diverse
Discovery of antagonistic Synechococcus-heterotrophic bacteria interactions
Bacteria-cyanobacteria associations involve nutrients exchange between the partners
Individual cell growth rates using "click chemistry"
Microbe-microbe interactions involving vitamin B1 exchange
Atomic force microscopy of bacterial surface membranes
Marine microbes rapidly turnover a high concentration of coral-spawn derived organic matter