Bacteria-cyanobacteria associations involve nutrients exchange between the partners

Measure individual cell carbon fixation of conjoint cyanobacteria and elemental fluxes between cyanobacteria and heterotrophic bacteria cells using NanoSIMS.

In our first stable isotope labeling experiments using natural microbial populations we did not find significant differences in 13C carbon and 15N nitrogen exchange in the free- versus cyanobacteria-associated heterotrophic bacteria. We are now optimizing culture conditions of the established co-culture system that we have developed with CC9311 (Synechococcus strain) and SWAT 3 and Pseudoalteromonas flavipulchra.

                                    Figure 2: NanoSIMS experiment: 15N and 13C individual cell uptake rate of Synechococcus cells and heterotrophic bacteria. In orange free Synechococcus,                                                                      in pink associated Synechococcus, in blue free heterotrophic bacteria, in green associated heterotrophic bacteria.

Other Project Data

Bacteria-cyanobacteria associations are phylogenetically diverse
Discovery of antagonistic Synechococcus-heterotrophic bacteria interactions
Bacteria-cyanobacteria associations involve nutrients exchange between the partners
Individual cell growth rates using "click chemistry"
Microbe-microbe interactions involving vitamin B1 exchange
Atomic force microscopy of bacterial surface membranes
Marine microbes rapidly turnover a high concentration of coral-spawn derived organic matter