Atomic force microscopy of bacterial surface membranes

The morphology of several marine bacterial isolates (Alteromonas sp. AltSIO, Pseudoalteromonas TW7, Vibrio SWAT-3, Flavobacterium BBFL7) (Figs. A,B,&C) and marine bacterial natural assemblages were extensively studied with AFM at different growth stages under differing conditions. Treatments included the addition of antibacterial agents such as silver nanoparticles and antibiotics. AFM data of the bacteria was collected to observe the cell structure and investigate formation of blebs and morphological changes at the nanoscale. AFM probing was also used to investigate the bio-mechanical properties of marine bacteria.

Raw AFM data is stored in Veeco Nanoscope format and available upon request to Dr. Azam 

Other Project Data

Bacteria-cyanobacteria associations are phylogenetically diverse
Discovery of antagonistic Synechococcus-heterotrophic bacteria interactions
Bacteria-cyanobacteria associations involve nutrients exchange between the partners
Individual cell growth rates using "click chemistry"
Microbe-microbe interactions involving vitamin B1 exchange
Atomic force microscopy of bacterial surface membranes
Marine microbes rapidly turnover a high concentration of coral-spawn derived organic matter